However, IVF using cryopreserved boar semen often results in lower fertilization rates than fresh semen, ,, , because boar sperm are susceptible to damage during cryopreservation and thawing. Frozen-thawed semen can be used repeatedly and also used for multiple experimental objectives. Semen can be used from a single ejaculate, thereby eliminating sperm source variability and improving consistency in experimental parameters. The number of sperm bound to oviduct cells was related to IVF polyspermy rates and may be more indicative of in vitro sperm function than traditional sperm motility and acrosome status evaluation.įrozen-thawed porcine semen has advantages over fresh and cooled semen when used for IVF. Our results indicate that post-thaw motility of frozen-thawed boar sperm is strongly related to acrosome integrity but has limited use for predicting IVF success. There was some relationship of post-thaw motility with IVF monospermic fertilization (P = 0.06, r 2 = 0.08) but not to other IVF outcomes.
The number of sperm bound to oviduct aggregates was correlated with IVF polyspermy rates (r 2 = 0.62, P 0.10). Among the sperm traits examined, sperm acrosome integrity was negatively correlated with post-thaw motility (r 2 = 0.64) but not with IVF results. Regression and correlation analyses were employed to determine the interrelationships between sperm traits and the relationships between post-thaw motility, sperm-oviduct binding and IVF outcomes. Each sample was tested by IVF in two to three independent replicates. Semen was thawed and motility was recorded microscopically and confirmed using computer-automated sperm assessment. Semen from 16 boars was cooled to 15 ☌ for overnight shipment before cryopreservation. Our objectives were to determine if post-thaw sperm motility, other traits that may be indicative of sperm function, or a novel assay of oviduct binding were related to IVF success. Samples that are below a designated motility threshold may be discarded. Semen samples are often screened for motility before use for IVF. However, the freezing and thawing processes result in compromised sperm function and IVF success. All rights reserved.Cryopreserved semen allows the use of single ejaculates for repeated analyses, potentially improving IVF consistency by eliminating interejaculate variability observed with fresh semen. It is concluded different combinations of commercial freezing extenders and thawing solutions have effects on the quality of cryopreserved boar semen in vitro.Ĭryopreservation Fertility Spermatozoa Swine Viability.Ĭopyright © 2021 Elsevier B.V. The use of T2, as compared with T1 thawing extender, resulted in an enhanced integrity of the plasma and acrosomal membranes (P = 0.008). The sperm plasma and acrosomal membrane integrity (AIMI) were greater (P = 0.009) when samples were preserved in F1 compared to F2 extender. Sperm thawed in T1 had a greater TMOT (P = 0.008) and PMOT (P = 0.033) at all times evaluated. The sperm progressive motility (PMOT) as time post-thawing increased was greater (P = 0.015) when dilutions occurred using F1 compared with F2 extender. There was no interaction between F × T × Time (P > 0.05), and no interaction between F × T (P > 0.05). The integrity assessments of the plasma and acrosomal membranes were performed at 30 and 360 min after thawing.
The sperm in diluted semen were evaluated for motility kinetics at 30, 180, and 360 min after thawing. Four straws from each treatment sample were thawed and diluted in T1 or T2, resulting in four treatments (F1-T1, F1-T2, F2-T1, and F2-T2). Samples were aliquoted in cryopreservation extender F1 or F2. Ejaculates were collected, diluted (1:1), and cooled before shipping at 17 ☌ overnight.
#Minitube cryoguard media androhep plus
This study was conducted to evaluate whether there were differences in viability of cryopreserved semen when using two different freezing (Minitube Cryoguard - F1 or Androstar® CryoPlus - F2) and thawing (Minitube Cryoguard Thawing solution - T1 or Androstar® Plus - T2) extenders.
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